DITHIONITROBENZOIC ACID

Synonyms. Dithionitrobenzoic acid; 2,2'-Dinitro-5,5'-dithiodibenzoesaeure; 2,2'-Dinitro-5,5'-dithiodibenzoic acid; 3,3'-Dithiobis(6-nitrobenzoic acid); Dithiobisnitrobenzoic acid; 3-Carboxy-4-nitrophenyl disulfide; 6,6'-Dinitro-3,3'-dithiodibenzoic acid; Bis(3-carboxy-4-nitrophenyl)disulfide; DTNB; Ellman's Reagent; 5-(3-Carboxy-4-nitro-phenyl)disulfanyl-2-nitrobenzoic acid; 5,5'-Disulfanediyl bis(2-nitrobenzoic acid);

DITHIONITROBENZOIC ACID

Click for original image

 

PRODUCT IDENTIFICATION

CAS RN

69-78-3

EINECS RN

200-714-4

FORMULA

 [SC6H3(NO2)COOH]2

MOLE WEIGHT

396.35

H.S CODE

 2930.90.2900

SMILES

 c1(c(ccc(c1)SSc1cc(c([N+](=O)[O-])cc1)C(O)=O)[N+](=O)[O-])C(O)=O

CLASSIFICATION

Indicator, Sulfhydryl reagent

EXTRA NOTES

A standard reagent for the determination of reactive sulfhydryl groups by absorbance measurements. It is used primarily for the determination of sulfhydryl and disulfide groups in proteins. The color produced is due to the formation of a thio anion, 3-carboxyl-4-nitrothiophenolate. [MeSH]

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE

off-white to yellow powder

MELTING POINT

240 ~ 245 C (Decomposes)

BOILING POINT

671 c

DENSITY

1.79 g/ml

SOLUBILITY IN WATER

 

SOLVENT SOLUBILITY

Soluble in ethanol, dioxane, phosphate

VAPOR DENSITY

 

log P(octanol-water)

  

VAPOR PRESSURE

  mm Hg

AUTOIGNITION TEMP

  
pK

 

REFRACTIVE INDEX

 (n20/D)

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions.

INCOMPATIBLE MATERIALS

Strong oxidizing agents, Strong bases

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 2, Flammability: 1, Reactivity: 0

 

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - Dithionitrobenzoic acid

Wikipedia Linking - Ellman's reagent

Google Scholar Search - Dithionitrobenzoic acid

U.S. National Library of Medicine - Dithionitrobenzoic acid

PubChem Compound Summary - Dithionitrobenzoic acid

NCBI (http://www.ncbi.nlm.nih.gov/) -  Dithionitrobenzoic acid

Material Safety Data Sheet - Dithionitrobenzoic acid

EPA - Substance Registry Services - Dithionitrobenzoic acid

http://www.sigmaaldrich.com/
Frequently called Ellman's Reagent or DTNB, this was originally developed for use as a sulfhydryl reagent. D8130 is tested for suitability for use as a reagent in sulfhydryl group determination. This aromatic disulfide reacts with aliphatic thiol groups to form a mixed disulfide of the protein and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group. DTNB has little if any absorbance, but when it reacts with -SH groups on proteins under mild alkaline conditions (pH 7-8), the 2-nitro-5-thiobenzoate anion (TNB2-) gives an intense yellow color at 412 nm.

 

SALES SPECIFICATION

APPEARANCE

off-white to yellow powder

ASSAY

99% min

WATER

1.0% max

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 

HAZARD CLASS

 

PACKING GROUP  

 

SAFETY INFORMATION

HAZARD OVERVIEW

GHS (Globally Harmonised System) Classification: Skin irritation. Eye irritation. Specific target organ toxicity - single exposure. Hazard statements: Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.

SIGNAL WORD Warning

PICTOGRAMS

PICTOGRAM_GHS07_exclamation.jpg

HAZARD STATEMENTS

H315-H319-H335

P STATEMENTS

P261-P264-P271-P280-P302 + P352-P304 + P340-P305 + P351 + P338-P312-P321- P332 + P313-P337 + P313-P362-P403 + P233-P405-P501

EC DIRECTIVES

 

HAZARD CODES

CODE_Xn_Xi_harmful_Irritant.gif

RISK PHRASES

36/37/38

SAFETY PHRASES

26-36

 

PACKING

Preserved in light-resistant and well-closed bottles

 

PRICE INFORMATION