HEMATOXYLIN

PRODUCT IDENTIFICATION
CAS NO. 517-28-2

HEMATOXYLIN 

EINECS NO. 208-237-3
FORMULA C16H14O6
MOL WT. 302.28

H.S. CODE

  

TOXICITY

  
SYNONYMS Haematoxylin; Natural Black 1; Haematoxylin; Hematoxylin;
(+)-Hematoxylin; cis-(+)-7,11b-Dihydrobenz(b)indeno(1,2-d)pyran-3,4,6a,9,10(6H)-pentol; (6aS,11bR)-7,11b-Dihydrobenz(b)indeno(1,2-d)pyran-3,4,6a,9,10(6H)-pentol; Hydroxybrazilin; Hydroxylrasilin; Hematoxyline; Hydroxybrasilin; (Other CAS RN: 1412-19-7; 22562-63-6;)
DERIVATION

 

CLASSIFICATION

  

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE brownish crystalline powder
MELTING POINT 200 C
BOILING POINT

 

SPECIFIC GRAVITY  
SOLUBILITY IN WATER soluble
pH  
VAPOR DENSITY

 

REFRACTIVE INDEX

 

NFPA RATINGS

 Health: 1 Flammability: 0 Reactivity: 1

AUTOIGNITION

 

FLASH POINT

 

STABILITY Stable under normal conditions.

GENERAL DESCRIPTION & APPLICATIONS

Hematoxylin is a brownish crystalline compound extracted from the heartwood of logwood. It is used as an indicator with a pH range of 5–6 and in the manufacture of ink, but is mainly used in oxidized form (called haematein) as a stain in microscopy. Hematoxylin is not itself the dye but conjuncted with a mordant, such as alum (ammonium, potassium, chrome. or ferric), ferric chloride, and phosphotungstic acid. These dyes are widely used as a stain in biology and medicine to demonstrate nuclear and cytoplasmic structures. These are also used to define and examine muscle fibers or connective tissue. The supply of hematoxylin is limited due to felling of forests. Some dyes are recommended as replacements such as Celestine blue (RN: 1562-90-9), Gallocyanine (CAS RN: 1562-85-2), Gallein (CAS RN: 2103-64-2), Solochrome cyanine R (CAS RN: 3564-18-9). But none of them are exactly recommended,  Brazilin (Natural red 24) extracted from the wood of the brazilwood and and sappanwood is very close to hematoxylin. The structure differ is having one less hydroxyl group. Brazilin has been also used as a dye for fabric, and to make inks. Brazilein is the oxidized form of  Brazilin. The oxidised brazilein is recommended as a substitute for haematein.

Hematoxylin staining preparation include:
  • Mayer’s Hematoxylin
  • Cole’s Hematoxylin
  • Harris hemalum
  • Phosphotungstic acid–hematoxylin ((PTAH))
  • Ehrlich's acid hematoxylin
  • Delafield's hematoxylin
  • hematoxylin-eosin
  • Harris hematoxylin
  • Heidenhain's iron hematoxylin
  • Weigert's iron hematoxylin
SALES SPECIFICATION

APPEARANCE

 brownish crystalline powder

SPECIFIC ABSORBTION

Е(1%/1sm)=166 (290 nm in acetonitrile)
WATER

6.5% max

pH RANGE

рН 5.0 (yellow)-рН 7.2 (violet)
TRANSPORTATION
PACKING
 
HAZARD CLASS  
UN NO.  
OTHER INFORMATION
Hazard Symbols: XI, Risk Phrases: 36/37/38, Safety Phrases: 26-36