FLUORESCEIN SODIUM

PRODUCT IDENTIFICATION

CAS NO. 518-47-8

FLUORESCEIN SODIUM 

EINECS NO. 208-253-0
FORMULA C20H12O5·2Na
MOL WT. 376.28
H.S. CODE 3204.12.5090
TOXICITY Oral rat LD50: 6721 mg/kg
SYNONYMS C.I. Acid Yellow 73; Soap yellow; C.I. 45350;
Water Soluble fluorescein; Uranine; Resorcinol Phthalein Sodium; Sodium fluoresceinate; Sodium hydroxy-o-carboxy-phenyl-fluorone; Disodium   3',6'-dihydroxyspiro[isobenzofuran-1(3H),9' -(9H)xanthene]-3-one; Fluorone; fluorescein, disodium salt; 9-o-carboxyphenyl-6-hydroxy -3-isoxanthone disodium salt; Sodium fluorescein; Disodium 6-hydroxy-3-oxo-9-xanthene-o-benzoate; Uranine WSS; Fluorescite; Flurenate; Uranine Yellow; Hydroxy-o-carboxy-phenyl-fluorone sodium salt; sodium fluoresceinate; Fluorescein W/S; 3',6'-Dihydroxy-spiro[3H-isobenzofuran-1,9'-xanthen]-3-one disodium salt; 9-(o-Carboxyphenyl)-3,6-dihydroxyxanthylium disodium salt; Spiro[isobenzofuran-1(3H),9'-[9H] xanthene]-3-one,3'6'-dihydroxy, disodium salt; C.I. 45350 disodium salt; Fluorescein sodium; Other RN: 3266-99-7, 31949-37-8, 34517-88-9, 41935-48-2, 121511-05-5, 208117-76-4, 574737-69-2, 893427-14-0
SMILES c1(c2c(oc3c1ccc(c3)[O-])cc(=O)cc2)c1c(cccc1)C(=O)[O-].[Na+].[Na+]

CLASSIFICATION

 

EXTRA NOTES

A phthalic indicator dye that appears yellow-green in normal tear film and bright green in a more alkaline medium, such as the aqueous humor, and is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma. It has been approved by FDA for use in externally applied drugs and cosmetics.

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE orange to red powder, odourless
MELTING POINT > 314 C
BOILING POINT  
SPECIFIC GRAVITY  
SOLUBILITY IN WATER Freely Souluble (600 g/l)
SOLVENT SOLUBILITY

MEG (500), DEG (550), PG (300), Glycerol (700), Methanol (230), Ethanol (45), Methoxyethanol (300)

pH 8 - 9.8
VAPOR DENSITY  
NFPA RATINGS Health: 1 Flammability: 0 Reactivity: 0
FLASH POINT

 

STABILITY Stable under ordinary conditions.

EXTERNAL LINKS & GENERAL DESCRIPTION

Wikipedia Linking - Fluorescein

Google Scholar Search - Fluorescein sodium

Drug Information Portal (U.S. National Library of Medicine) - Fluorescein sodium

PubChem Compound Summary - Fluorescein sodium

KEGG (Kyoto Encyclopedia of Genes and Genomes) - Fluorescein sodium

http://www.ebi.ac.uk/Fluorescein sodium

http://www.ncbi.nlm.nih.gov/ - Fluorescein sodium

http://toxnet.nlm.nih.gov/
Hazardous Substances Data Bank - Fluorescein sodium

Local
Fluorescein is a common fluochrome dye; a yellowish to red crystalline powder; insoluble in water; soluble in dilute acids, and dilute alkali. It is obtained from the condensation of phthalic anhydride with resorcin on heating. It is used in dying silk and wool. It is the parent compound of eosin. All eosins are bromine derivatives of fluorescin, used in dyeing textiles, ink manufacturing, in coloring cosmetics, in coloring gasoline and as a toner. Eosin is a composition of Wright stain, in modified Nocht's stain and eosin-methylene blue medium. The sodium salt of fluorescein is called uranine which are used in dying silk and wool, in oceanography as a marker in sea water, and in coloring agrochemicals, fertilizers, detergents and antifreezers. Fluorescein sodium (uranine) is an orange-red crystalline powder; odorless, water-soluble. In biology, it is used as a detection agent for visible images of structures or details which are not seen by eyes. It is used as a diagnostic indicator in assessing corneal trauma and fitting contact lenses. It is used retinal angiography detect latent blood stains for the study of antigen-antibody reactions in serum. The isothiocyanate salt of fluorescein is capable of being conjugated to amine groups of proteins inside cells, particularly antibodies, and hence used to label and track the molecules in various assays.

SALES SPECIFICATION

APPEARANCE

orange to red powder, odourless

DYE CONTENT

96.0 - 100.5% ( dry basis)

PURITY BY HPLC

96.0% min

WATER

5.0% max

SULFATE

1.2% max

pH

7 - 9 (2% solution)

CHLORIDE

0.4% max

ZINC

passes test

TRANSPORTATION
PACKING  
HAZARD CLASS Not regulated
UN NO.  
SAFETY INFORMATION

HAZARD OVERVIEW

Irritating to eyes. May cause skin and respiratory tract irritation.

GHS

 

SIGNAL WORD Warning

PICTOGRAMS

HAZARD STATEMENTS

H319

P STATEMENTS

P280-P305 + P351 + P338

EC DIRECTIVES

 

HAZARD CODES

RISK PHRASES

36

SAFETY PHRASES

26-37/39

BIOLOGICAL STAINS, INDICATORS