PRODUCT IDENTIFICATION

CLASSIFICATION

Buffer, Alkalize, Antibacterial, Pharmaceutical Vehicle, Excipient, Good's buffer, Amino alcohol

EXTRA NOTES

 An organic amine proton acceptor.
EPA Pesticide Chemical Code 083901 

PHYSICAL AND CHEMICAL PROPERTIES

REFRACTIVE INDEX

EXTERNAL LINKS & GENERAL DESCRIPTION

Wikipedia Linking

Google Scholar Search

Drug Information Portal (U.S. National Library of Medicine) - Tris

PubChem Compound Summary - Tromethamine

http://www.ebi.ac.uk/chebi/ -  Tromethamine

http://www.ncbi.nlm.nih.gov/ -  Tromethamine

Material Safety Data Sheet

http://www.sigmaaldrich.com/
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

http://msdssearch.dow.com/
TRIS AMINO is well-known worldwide as a buffer in biochemical reactions. It can be used to buffer anywhere within the pH range of 7 to 9 by utilizing a combination of the free base and its salts; the hydrochloride salt is especially popular for this purpose. In fact, a buffer solution containing TRIS AMINO and its hydrochloride salt at a molar ratio of 1:3 is considered a standard buffer solution for use in the physiological pH range of 7.3 to 7.5

Local: Buffer is a substance, generally a solution, that can keep its pH constant, despite the addition of strong acids or strong bases and external influences of temperature, pressure, volume, redox potential. Buffer prevents change in the concentration of another chemical substance, e.g., proton donor and acceptor systems that prevent marked changes in hydrogen ion concentration (pH). Many acid-base reactions take place in living organisms. However, for organisms to perform certain vital functions, the body fluids associated with these functions must maintain a constant pH. For example, blood must maintain a pH of close to 7.4 in order to carry oxygen from the lungs to cells; blood is therefore a powerful buffer. The commonest buffer in chemical solution systems is the acid-base buffer.

• Bicarbonate buffer; a buffer system composed of bicarbonate ions and dissolved carbon dioxide; in the body, this system is an important factor in determining the pH of the blood as the concentration of bicarbonate ions is regulated by the kidneys and of carbon dioxide by the respiratory system.
• Cacodylate buffer; one containing an organic arsenical salt, used in preparing fixatives for electron microscopy.
• Phosphate buffer,   a buffer system composed of KH₂PO₄ and Na₂HPO₄; in the body, it is important in regulating the pH of the renal tubular fluids; when 0.025 molal (equimolal of the potassium and sodium salts), the pH is 6.865 at 25 C.
• Protein buffer,   a buffer system involving proton donor and proton acceptor groups of the amino acid residues of proteins.
• TRIS buffer (tromethamine): an amine base used intravenously as an alkalizer for the correction of metabolic acidosis. The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per C temperature decrease, and decreases 0.03-0.05 unit per ten-fold dilution.
• Veronal buffer; a barbital buffer commonly used in the preparation of fixatives for electron microscopy.

Tromethamine is used as an intermediate for the preparation of surface active agents, vulcanization accelerators, and pharmaceuticals, and used as a titrimetric standard.

APPEARANCE

99.9% min

168 - 172 C

WATER INSOLUBLES

0.1% max

Cl

5ppm max

LOSS ON DRYING

0.5% max

HEAVY METALS

5ppm max

CLARITY

clear (in 40% water)

pH

10 - 11.5

SULFATE

5ppm max

HAZARD OVERVIEW

GHS

PICTOGRAMS

HAZARD STATEMENTS

H315:  Causes skin irritation
H335:  May cause respiratory irritation
H319:  Causes serious eye irritation

PRECAUTIONARY STATEMENTS

P261:  Avoid breathing dust/fume/gas/mist/vapors/spray
P280:  Wear protective gloves/protective clothing/eye protection/face protection
P302 + P352:  IF ON SKIN: Wash with plenty of soap and water
P305 + P351 + P338:  IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing

Xi Irritant

RISK PHRASES

36/37/38:  Irritating to eyes, respiratory system and skin.

SAFETY PHRASES

26:  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
37/39:  Wear suitable gloves and eye/face protection.