7-AMINOACTINOMYCIN D

Synonyms. 2,7-Diamino-4,6-dimethyl-3-oxo-N,N'-bis[(3R,6S,7R,16S)-7,11,14-trimethyl-2,5,9,12,15- pentaoxo - 3,10 -di(propan-2-yl)- 8-oxa-1,4,11,14-tetrazabicyclo[14.3.0]nonadecan-6-yl]phenoxazine- 1,9-dicarboxamide; 7-AAD; 7-Amino-AMD; Flu 402;

7-AMINOACTINOMYCIN D

 

PRODUCT IDENTIFICATION

CAS RN

7240-37-1

EINECS RN

256-342-8, 232-693-2 

FORMULA

C62H87N13O16

MOLE WEIGHT

1270.43

H.S CODE

 2941.90.1010

SMILES

 C1(O[C@@H](C)[C@@H](C(N[C@@H](C(N2[C@@H](C(=O)N(CC(N([ C@@H]1C (C)C) C)=O)C)CCC2 )=O)C(C)C)=O)NC(c1c2nc3c(oc2c(c(c1 )N)C) c (c(=O)c (c3C (N[C@ @H]1[C@@H] (OC([C@@H](N(C(CN(C(=O) [C@@H]2N(C([ C@@H](NC1 =O)C(C)C)= O)CCC2)C) =O)C)C(C)C)= O)C)= O) N)C)=O)=O

CLASSIFICATION

Fluorescent dye, Polypeptide antibiotic

EXTRA NOTES

 Staining reagent used in flow cytometry. Fluorescent DNA stain

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE.

dark purple powder

MELTING POINT

245 - 250 C

BOILING POINT

 

DENSITY

 

SOLUBILITY IN WATER

 

SOLVENT SOLUBILITY  

VAPOR DENSITY

 

log P(octanol-water)

  

VAPOR PRESSURE

  

AUTOIGNITION TEMP

  
pH

 

REFRACTIVE INDEX

 

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions.

INCOMPATIBLE MATERIALS

Strong acids, Strong bases, Strong oxidizing agents, Exposure to moisture

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 4,Flammability: 0, Reactivity: 1

 

EXTERNAL LINKS & GENERAL DESCRIPTION

Wikipedia Linking - 7-Aminoactinomycin D

Google Scholar Search - 7-Aminoactinomycin D

Drug Information Portal (U.S. National Library of Medicine) - 7-Aminoactinomycin D

PubChem Compound Summary - 7-Aminoactinomycin D7-Aminoactinomycin D

http://www.ncbi.nlm.nih.gov/ - 7-Aminoactinomycin D

http://www.sigmaaldrich.com/
7-AAD is used in flow cytometry analysis of viable cells. Cell surface markers were stained by FITC and phycoerythrin-conjugated antibody. After surface staining, cells were further stained with 10 μg/ml of 7-AAD in PBS on ice for 30 minutes. After washing with PBS twice, the cells were fixed in 1% paraformaldehyde supplemented with 50 μg/ml actinomycin D. Non-apoptotic cells are 7-AAD negative.

Local:
Actinomycin D is a compound composed of a two cyclic peptides attached to a phenoxazine that is derived from bacteria of the genus Streptomyces. It is a DNA-intercalator with growth-inhibitory properties. It binds to DNA at the transcription initiation complex and inhibits elongation by RNA polymerase. Actinomycin-D is one of the old apoptosis inducer drugs which has been used in anti-cancer activity, but is not normally used as due to high toxicity causing damage to genetic material. 7-Aminoactinomycin D is the fluorescent derivative having strong affinity for DNA, used as stains in staining for imaging DNA fluorescence. It has a very large emission range with most blue and green fluorophores compatiblity and even red fluorophores.

 

SALES SPECIFICATION

APPEARANCE

dark purple powder

PURITY 97.0% min

SOLUBILITY

clear red (in methanol, chloroform)

WATER CONTENT 0.5% max

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

3462

HAZARD CLASS

 6.1
PACKING GROUP II

 

SAFETY INFORMATION

HAZARD OVERVIEW

Highly Toxic (USA). Very toxic by inhalation, in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin. Possible sensitizer. Target organ(s): Liver. Bone marrow. Light sensitive. Moisture sensitive. Cause eye irritation. Causes skin irritation. May be harmful if absorbed through the skin. May cause irritation of the digestive tract. May be harmful if swallowed. Causes respiratory tract irritation. May be harmful if inhaled.

GHS

  

SIGNAL WORD

Danger

PICTOGRAMS

HAZARD STATEMENTS

H300-H310-H315-H319-H330-H335

P STATEMENTS

P260-P264-P280-P284-P301 + P310-P302 + P350

EC DIRECTIVES

 

HAZARD CODES

T+ Very toxic

RISK PHRASES

 26/27/28-36/37/38

SAFETY PHRASES

 26-28-36/37-45

 

PACKING