FLUORESCEIN-5-ISOTHIOCYANATE

Synonyms. Fluorescein-5-isothiocyanate; Fluorescein isothiocyanate isomer I; 2-(6-Hydroxy-3-oxo-(3H)-xanthen-9-yl)-5-isothiocyanatobenzoic acid; 3',6'-Dihydroxy-5-isothiocyanato-spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one; 5-Fluorescein Isothiocyanate; 6-isothiocyanato-3',6'-bis(oxidanyl)spiro(2-benzofuran-3,9'-xanthene)-1-one;

FLUORESCEIN-5-ISOTHIOCYANATE

Click for original image

 

PRODUCT IDENTIFICATION

CAS RN

3326-32-7

EINECS RN

222-042-0

FORMULA

C21H11NO5S

MOLE WEIGHT

389.38

H.S CODE

 2938.90.0000

SMILES

 C12(c3c(cc(N=C=S)cc3)C(O2)=O)c2c(Oc3c1ccc(c3)O)cc(O)cc2

CLASSIFICATION

Coloring agent, Fluorescent dye, Indicator

EXTRA NOTES

Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques. [MeSH]
Other RN: 55177-69-0, 86151-70-4, 113394-21-1, 122655-66-7, 63469-13-6 (hydrochloride)

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE.

orange powder

MELTING POINT

> 360 C

BOILING POINT

735 C

DENSITY

1.542

SOLUBILITY IN WATER

practically insoluble

SOLVENT SOLUBILITY

Soluble in DMSO.

VAPOR DENSITY

 

log P(octanol-water)

  

VAPOR PRESSURE

  

AUTOIGNITION TEMP

  
pK

 

REFRACTIVE INDEX

1.715

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions. Moisture Sensitive

INCOMPATIBLE MATERIALS

Alcohols, Strong bases, Amines, acids, Strong oxidizing agents

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 3, Flammability: 0, Reactivity: 0

 

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - Fluorescein-5-isothiocyanate

Wikipedia Linking - Fluorescein isothiocyanate

Google Scholar Search - Fluorescein-5-isothiocyanate

U.S. National Library of Medicine - Fluorescein-5-isothiocyanate

PubChem Compound Summary - Fluorescein-5-isothiocyanate

ChEBI (http://www.ebi.ac.uk/chebi/) -  Fluorescein-5-isothiocyanate

NCBI (http://www.ncbi.nlm.nih.gov/) -  Fluorescein-5-isothiocyanate

Material Safety Data Sheet - Fluorescein-5-isothiocyanate

EPA - Substance Registry Services - Fluorescein-5-isothiocyanate

http://www.sigmaaldrich.com/
Application: Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.

 

SALES SPECIFICATION

APPEARANCE

orange powder

ASSAY

95% min

WATER

0.5% max

ABSORPTION

488 ~ 494 nm (λmax 0.1N NaOH)

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 

HAZARD CLASS

 

PACKING GROUP  

 

SAFETY INFORMATION

HAZARD OVERVIEW

GHS (Globally Harmonised System) Classification: Respiratory sensitisation. Skin sensitisation. Hazard statements: May cause an allergic skin reaction. May cause allergy or asthma symptoms or breathing difficulties if inhaled.

SIGNAL WORD Danger

PICTOGRAMS

HAZARD STATEMENTS

H317-H334

P STATEMENTS

P261-P280-P342 + P311

EC DIRECTIVES

 

HAZARD CODES

RISK PHRASES

42/43

SAFETY PHRASES

22-36/37-45

 

PACKING

Preserve in light-resistant and well-closed containers

 

PRICE INFORMATION