N-HYDROXYSUCCINIMIDOBIOTIN

Biotinyl-N-Hydroxysuccinimide ester; BNHS, NHS-D-Biotin; N-Succinimide-D-Biotinate; Biotinyl-N-hydroxy-succinimide; Biotin O-Su; (2,5-Dioxopyrrolidin-1-yl)5-(2-oxo-1,3,3a,4,6,6a- hexahydrothieno[3,4-d]imidazol-6-yl)pentanoate; 1-((5-(2-Oxohexahydro-1H-thieno(3,4-d) imidazol-4-yl)pentanoyl)oxy)-2,5-pyrrolidinedione; Biotin-NHS; Biotin-OSu;

N-HYDROXYSUCCINIMIDOBIOTIN

 

PRODUCT IDENTIFICATION

CAS RN

35013-72-0

EINECS RN

 

FORMULA

C14H19N3O5S

MOLE WEIGHT

341.38

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE

White to off-white powder

MELTING POINT

212 C

BOILING POINT

 

DENSITY

 

SOLUBILITY IN WATER

 

pH

 

VAPOR DENSITY

 

REFRACTIVE INDEX

 

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions

INCOMPATIBLE MATERIALS

Strong oxidizing agents.

DECOMPOSITION PRODUCTS

Carbon oxides. nitrogen oxides, Sulphur oxides.

POLYMERIZATION Has not been reported

NFPA RATINGS

Health: 0 Flammability: 0 Reactivity: 0

 

SAFETY

HAZARD NOTES

Causes irritation. Avoid contact with eyes, skin, and clothing.

EYE

May cause eye irritation.

SKIN

May be harmful if absorbed through skin. May cause skin irritation.

INGESTION

May be harmful if swallowed.

INHALATION

May be harmful if inhaled. May cause respiratory tract irritation.

CHRONIC

 

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 
HAZARD CLASS

 

PACKING GROUP

 

HAZARD SYMBOL

 

RISK PHRASES

 

SAFETY PHRASES

22-24/25

 

OTHER INFORMATION

Proteomic approaches require simple and efficient protein purification methodologies that are amenable to high throughput. Biotinylation is an attractive approach for protein complex purification due to the very high affinity of avidin/streptavidin for biotinylated templates. Here, we describe an approach for the single-step purification of transcription factor complex(es) based on specific in vivo biotinylation. (http://www.pnas.org/)

Biotinylation is a rapid method of detecting nucleic acids for use in a Western blot. Biotin is covalently coupled to primary amines of immunoglobin. Biotinylation of antigens is used to improve immunoassays. Proteins can be biotinylated in 1 hour. CHO B2 cells transfected with alpha5 were washed with buffer and biotinylated for 60 minutes. After washing, the cells were extracted with protease inhibitors for 30 minutes, and centrifuged for 10 minutes. (http://student.biology.arizona.edu/)

Biotinylation of a protein generally involves chemical modification of a translated protein. Using this methodology, however, biotinylation at a specific position remains difficult. We investigated whether it would be possible to use an Escherichia coli initiator tRNAfmet aminoacylated with methionine biotinylated at the .ALPHA.-amino group to introduce a biotin tag specifically at the N terminus. We report here that a biotin tag could be incorporated into the green fluorescent protein (GFP) at the N-terminal site, in the presence of an E. coli initiator tRNAfmet aminoacylated with methionine biotinylated at the .ALPHA.-amino group. The biotinylated GFP was purified by simple monomeric streptavidin-agarose affinity column chromatography. Based on the total amount of GFP molecules, the purification yield and the biotin labelling efficiency of this system were approximately 7% and 10-20%, respectively, according to the densitometric analysis of Western blots. Judging from the results of a fluorescence imaging experiment, almost all the purified GFP molecules retained the native fluorescence activity. Importantly, the present results support the hypothesis that the E. coli initiator tRNAfmet aminoacylated with a relatively large substituent can be recognized by an E. coli ribosome and adequately placed at the P site to initiate translation. (http://sciencelinks.jp/)

 

 

SALES SPECIFICATION

APPEARANCE

White to off-white powder

PURITY

99.0% min

 

PRICE INFORMATION