PYRONINE

Synonyms. Pyronine; Pyronine G; Pyronine Y; C.I. 45005; Methyl pyronin; 3,6-Bis(dimethylamino) xanthylium chloride; (6-(Dimethylamino)- 3H-xanten-3-ylidene)dimethyl ammonium chloride; N-(6-(Dimethylamino)-3H-xanthen-3-ylidene)-N-methyl methanaminium chloride; Pyronin G; Pyronin Y; Pyronin Yellow; Tetramethyl pyronin; Pyronine ZH;

Pyronine

Click for original image

 

PRODUCT IDENTIFICATION

CAS RN

92-32-0

EINECS RN

202-147-8

FORMULA

C17H19ClN2O

MOLE WEIGHT

302.80

H.S CODE

 3204.19.4000

SMILES

 c12c(cc3ccc(\cc3o1)=[N+](\C)C)ccc(c2)N(C)C.[ClH-]

CLASSIFICATION

Coloring Agent, Cationic xanthene dye

EXTRA NOTES

Xanthene dye used as a bacterial and biological stain. Synonyms: Pyronin; Pyronine G; Pyronine Y. Use also for Pyronine B. which is diethyl-rather than dimethylamino-.
Other RN: 20195-95-3, 61260-70-6, 115624-92-5, 167382-93-6

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE.

dark green powder

MELTING POINT

250 ~ 260 C

BOILING POINT

 

DENSITY

 

SOLUBILITY IN WATER

1 mg/ml

SOLVENT SOLUBILITY

EGME 9 mg/ml, EtOH 3 mg/ml

VAPOR DENSITY

 

log P(octanol-water)

 1

VAPOR PRESSURE

  

AUTOIGNITION TEMP

  
pK

 

REFRACTIVE INDEX

 

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions.

INCOMPATIBLE MATERIALS

Strong oxidizing agents

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 1, Flammability: 0, Reactivity: 0

 

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - Pyronine

Wikipedia Linking - Xanthene

Google Scholar Search - Pyronine

Drug Information Portal (U.S. National Library of Medicine) - Pyronine

PubChem Compound Summary - Pyronine

KEGG (Kyoto Encyclopedia of Genes and Genomes) -  Pyronine

http://www.ncbi.nlm.nih.gov/ -  Pyronine

Material Safety Data Sheet - Pyronine

EPA - Substance Registry Services - Pyronine

http://www.sigmaaldrich.com/
Pyronin Y solutions are red in transmitted light, displaying a yellow flouresence in reflected light. Pyronin Y is used widely in combination with methyl green to selectively and differentially stain nucleic acids. In the dichromatic dye solution, Pyronin Y stains RNA red, while methyl green stains the DNA green. Transfer RNA (tRNA) will complex with Pyronin Y at a 1 : 10 molar ratio when analyzed by polyacrylamide gel electrophoresis. tRNA (4 to 8 μg) when complexed with Pyronin Y is visible as a red band during electrophoresis. Pyronin Y can also be used in correlating RNA content by flow cytometry. The excitation maximum of Pyronin Y is between 488 nm and 530 nm. The emission maximum of RNA bound Pyronin Y is 570 nm. In addition, Pyronin Y solutions may be utilized in staining liver cells that have been fixed in Carnoy's fluid.

 

SALES SPECIFICATION

APPEARANCE

dark green powder

DYE CONTENT

50% min

ABSORPTION

546 ~ 551 nm (in 50% ethanol) λ max

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 

HAZARD CLASS

 

PACKING GROUP  

 

SAFETY INFORMATION

HAZARD OVERVIEW

Not a dangerous substance or mixture according to GHS (Globally Harmonised System). Eyes: May cause eye irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Ingestion: May be harmful if swallowed.

HAZARD CODES

 

RISK PHRASES

 

SAFETY PHRASES

24/25-37

 

PACKING

Preserve in light-resistant and well-closed containers