THIAZOLYL BLUE

Synonyms. Thiazolyl blue; 3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide; 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide; 2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide; Methylthiazoletetrazolium; MMT Tetrazolium; Thiazolyl Blue Monotetrazolium; Thiazolyl blue tetrazolium bromide; 2-(3,5-Diphenyltetrazol-2-ium-2-yl)-4,5-dimethyl-1,3-thiazole bromide;

THIAZOLYL BLUE

Click for original image

 

PRODUCT IDENTIFICATION

CAS RN

298-93-1

EINECS RN

206-069-5

FORMULA

C18H16BrN5S

MOLE WEIGHT

414.32

H.S CODE

 2934.10.2000

SMILES

 c1cc(c2nn(c3ccccc3)[n+](c3sc(c(n3)C)C)n2)ccc1.[BrH-]

CLASSIFICATION

Coloring agent

EXTRA NOTES

Tetrazolium salts are widely used for detecting redox potential of cells for viability, cytotoxicity and proliferation assays. ... Reduction of MTT remains the most common assay for tetrazolium salt�based viability testing. [HSDB]
Other RN: 2348-71-2, 3568-69-2, 61357-96-8, 74722-46-6, 85556-98-5, 93550-34-6, 117419-96-2, 117830-92-9, 133683-46-2, 156758-88-2, 460315-48-4, 13146-93-5 (parent)

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE.

yellow crystalline powder

MELTING POINT

195 C (Decomposes)

BOILING POINT

 

DENSITY

 

SOLUBILITY IN WATER

5 mg/ml

SOLVENT SOLUBILITY

 

VAPOR DENSITY

 

log P(octanol-water)

  

VAPOR PRESSURE

  

AUTOIGNITION TEMP

  
pK

 

REFRACTIVE INDEX

 

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions.

INCOMPATIBLE MATERIALS

Strong oxidizing agents

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 2, Flammability: 0, Reactivity: 0

 

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - Thiazolyl blue

Google Scholar Search - Thiazolyl blue

U.S. National Library of Medicine - Thiazolyl blue

PubChem Compound Summary - Thiazolyl blue

http://www.ebi.ac.uk/chebi/ -  Thiazolyl blue

http://www.ncbi.nlm.nih.gov/ -  Thiazolyl blue

Hazardous Substances Data Bank - Thiazolyl blue

EPA - Substance Registry Services - Thiazolyl blue

http://www.sigmaaldrich.com/
Application: Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemica reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

 

SALES SPECIFICATION

APPEARANCE

yellow crystalline powder

ASSAY

98% min

ABSORPTION MAX

239 ~ 247 nm

MELTING POINT

190 C

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 

HAZARD CLASS

 

PACKING GROUP  

 

SAFETY INFORMATION

HAZARD OVERVIEW

GHS (Globally Harmonised System) Classification: Skin irritation. Eye irritation. Germ cell mutagenicity. Specific target organ toxicity - single exposure. Hazard statements: Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation. Suspected of causing genetic defects.

SIGNAL WORD Warning

PICTOGRAMS

HAZARD STATEMENTS

H315-H319-H335-H341

P STATEMENTS

P261-P281-P305 + P351 + P338

EC DIRECTIVES

 

HAZARD CODES

RISK PHRASES

36/37/38

SAFETY PHRASES

26-36

 

PACKING

Preserve in light-resistant and well-closed containers

 

PRICE INFORMATION