TRYPAN BLUE

Synonyms. Trypan Blue; C.I. Direct Blue 14; C.I. Direct Blue 14 tetrasodium salt; C.I. 23850; Trypan (Congo) Blue; Trypanblau; Vision Blue; Tetrasodium 3,3'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(azo)) bis(5-amino-4-hydroxynaphthalene- 2,7-disulphonate; Azidinblau 3B; Azidine Blue 3B; Azurro diretto 3B; Bencidal Blue 3B; Benzamine Blue; Benzanil Blue R; Benzo Blue 3B; Bleu diamine; Bleu trypane N; Brasilamina Blue 3B; Centraline Blue 3B; Chloramine Blue 3B; Chlorazol Blue 3B; Congo Blue; Cresotine Blue 3B; Diamine Blue; Dianil Blue; Diaphtamine Blue TH; Diazine Blue 3B; Diazol Blue 3B; Diphenyl Blue 3B; Modr Trypanova; Naphthylamine Blue; Niagara Blue; Pyrazol Blue 3B; Sodium ditolyl-diazobis-8-amino-1-naphthol-3,6-disulfonate; Tripan Blue; Niagara Blue 3B;

TRYPAN BLUE

Click for original image

 

PRODUCT IDENTIFICATION

CAS RN

72-57-1

EINECS RN

200-786-7

FORMULA

C34H24N6O14S4Na4

MOLE WEIGHT

960.81

H.S CODE

 3204.14.5000

SMILES

 c12c(cc(c(c1O)/N=N/c1c(cc(cc1)c1cc(c(cc1)/N=N/c1c(cc3c(c1O) c(cc(c 3)S (=O)(=O)[O-])N)S(=O)(=O)[O-])C)C)S(=O)(=O)[O-]) cc(cc2N)S(=O) (=O) [O-].[Na+].[Na+].[Na+].[Na+]

CLASSIFICATION

Coloring Agent. Azo dye

EXTRA NOTES

Overall Carcinogenic Evaluation: Group 2B.
A diazo-naphthalene sulfonate that is widely used as a stain.
Trypan blue is a blue acid dye that contains two azo chromophores. It is a large, hydrophilic, tetrasulfonated dye.Trypan blue has been used as a vital stain as it is excluded by most living cells. It has been used to stain collagen and amyloid. It is also used in chlamydospore agar for fungi. When Trypan blue binds to proteins the resulting complex emits red fluorescence. (sigmaaldrich)

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE.

dark blue to black powder

MELTING POINT

>280 C

BOILING POINT

 

DENSITY

 

SOLUBILITY IN WATER

10 g/l

SOLVENT SOLUBILITY

Insoluble in alcohol. Soluble in cellosolve, ethylene glycol, acids

VAPOR DENSITY

 

log P(octanol-water)

 -0.12

VAPOR PRESSURE

  

AUTOIGNITION TEMP

  
pK

 

REFRACTIVE INDEX

 

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions.

INCOMPATIBLE MATERIALS

Strong oxidizing agents

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 0, Flammability: 0, Reactivity: 0

 

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - Trypan Blue

Wikipedia Linking - Trypan Blue

Google Scholar Search - Trypan Blue

IPCS INCHEM -  Trypan Blue

Drug Information Portal (U.S. National Library of Medicine) - Trypan Blue

PubChem Compound Summary - Trypan Blue

KEGG (Kyoto Encyclopedia of Genes and Genomes) - Trypan Blue

http://www.ncbi.nlm.nih.gov/ - Trypan Blue

http://toxnet.nlm.nih.gov/
Hazardous Substances Data Bank - Trypan Blue

EPA - Substance Registry Services - Trypan Blue

 

SALES SPECIFICATION

APPEARANCE

dark blue to black powder

DYE CONTENT

80% min

ASORPTION

607 nm (λ max in MeOH), 588 nm (λ max in H2O)

LOSS ON DRYING

8% nax

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 

HAZARD CLASS

 
PACKING GROUP  

 

SAFETY INFORMATION

HAZARD OVERVIEW

OSHA Hazards:Carcinogen, Target Organ Effect, Teratogen. Target Organs:Liver. May cause cancer.

SIGNAL WORD Danger

PICTOGRAMS

HAZARD STATEMENTS

H350

P STATEMENTS

P201-P233-P308 + P313

EC DIRECTIVES

 

HAZARD CODES

RISK PHRASES

45

SAFETY PHRASES

45-53

 

PACKING

Preserve in light-resistant and well-closed containers